云南灯盏花遗传变异的RAPD分析

RAPD Analysis on the Genetic Variation of Erigeron breviscapus from Yunnan

运用RAPD (randomamplifiedpolymorphicDNA)技术对云南灯盏花 (Erigeronbrevis capus) 6个居群进行分析 ,研究其遗传变异及遗传结构 ,用外类群紫菀对比分析其亲缘关系。随机挑选 16个引物在 6个居群中共产生 2 33条DNA片段 ,其中 192条带具有遗传多态性 ,约占 82 4 0 %。多态位点百分比PPB、等位基因数A、有效等位基因数Ae、Nei’s基因多样性指数H和Shannon多样性指数I在物种水平分别为 82 4 0 %、 1 82 4 0、 1 30 0 5、0 1896、 0 30 2 1;居群的平均值分别为 5 4 2 3%、 1 5 40 1、 1 2 6 91、 0 16 0 7、 0 2 4 6 0。灯盏花的遗传多样性较丰富 ,基因分化系数Gst值为 0 346 0 ,有 6 5 4 0 %的变异来自居群内 ,6个居群的遗传一致度较高 ,在 0 90 37～ 0 972 3之间 ,平均为 0 94 10。利用UPGMA对 6居群聚类分析 ,结果表明 :丘北居群 (YB)和文山居群 (YX)亲缘关系最近 ,小哨居群 (Y )和新平居群 (YA)次之 ,丘北居群 (YB)和腾冲居群 (ZA)亲缘关系最远。

Random amplified polymorphic DNA (RAPD) was amplified to studied genetic diversity and genetic structure of 6 populations of Erigeron breviscapus in Yunnan province.As a contrast,one population of Aster himalaicus was selected to study their phylogenetic relationship.A total of 16 primers were screened to use,and 233 bands were amplified,among which 192 (82.40%) were polymorphic.At specific level,the percentage of polymorphic bands PPB,number of alleles A,effective number of alleles Ae,Nei's gene diversity H,and Shannon's Information index I were 82.40%,1.8240,1.3005,0.1896,and 0.3021 respectively,and at population level were on average 54.23%,1.5401,1.2691,0.1607,and 0.2460 respectively.G_(st) was 0.3460,meaning that 65.40% of the genetic variation was found within the populations.The genetic identity among the populations was high,ranging from 0.9037 to 0.9723 and averaging 0.9410.An analysis of 6 populations using UPGMA showed that,there was the closest relationship between Qiubei (YB) population and Wenshan (YX) population,then between Xiaoshao (Y) and Xiping (YA) populations,and the last between Wenshan (YX) population and Tengchong (ZA) population.The genetic distance is almost positively related to the spatial or geographical distance between the populations.