摘要
目的建立测定参茜固经颗粒(党参、茜草、地黄、女贞子等)中大叶茜草素含量的高效液相色谱方法。方法采用安捷伦ExtendC18(250mm×4.6mm,5μm)柱,以甲醇水四氢呋喃(310∶90∶3)为流动相,流速1.0mL·min-1;在249nm波长处检测。结果大叶茜草素在0.056～0.29μg范围内呈线性关系,r=0.9999;平均加样回收率为99.36,RSD=1.60(n=5)。结论该方法简便准确,重现性好,可用于测定参茜固经颗粒中的大叶茜草素含量。
AIM: To establish a HPLC for the determination of rubimaillin in Shenqiangujing Granules (Radix Codonopsis, Radix Rubiae, Radix Rehmanniae, Fructus Ligustri Lucidi, etc.) METHODS: HPLC column was C-18 (250 mm×4.6 mm, 5 μm). The mobile phase was methanol-water-tetrahydrofuran (310∶90∶3). Flow rate was 1.0 mL·min -1 . The detection wavelength was at 249 nm. RESULTS: The linear range of rubinaillin was in the range of 0.056～0.29 μg, r=0.9999. The average recovery was 99.36% (RSD=1.60%, n=5). CONCLUSION: The method is accurate with the good reproducibility, and can be used for the determination of rubimaillin in Shenqiangujing Granules.
出处
《中成药》
CAS
CSCD
北大核心
2005年第1期28-31,共4页
Chinese Traditional Patent Medicine
