银杏叶提取物GBE50对兔心肌组织bcl-2、bcl-xL基因表达的影响

Effect of gene expression of bcl-2 and bcl-xL in rabbit myocardium by Ginko biloba extract GBE50

目的探讨新型银杏叶提取物GBE50对兔心肌细胞抗凋亡基因bcl2、bclxL表达的影响。方法成年雄性新西兰大白兔随机分为GBE50组和对照组,GBE50组每日按体重100mg·kg-1口饲给予新型银杏叶提取物GBE50,对照组仅给予赋形剂2羧甲基纤维素钠,饲养4周后,处死动物,取左室心肌组织,采用RTPCR技术在凝胶电泳成像基础上半定量检测bcl2、bclxL基因的表达水平。结果GBE50组bcl2、bclxL基因的表达显著高于对照组(P<0.05)。结论新型银杏叶提取物GBE50可促进兔心肌细胞抗凋亡基因bcl2、bclxL的表达。

AIM: To investigate affection of the novel Ginko biloba extract GBE50 on bcl-2、bcl-xL gene expression in rabbit myocardium. METHODS: Adult male New Zealand rabbits were randomly divided into 2 groups. GBE50 group were orally administrated with 100 mg/kg GBE50 daily. Control group were only administrated vehicle 2% CMC-Na. After being raised for 4 weeks, the animals were killed and the ventricular tissue were excised. We performed reverse transcriptase polymerase (RT-PCR) to measure gene expression of bcl-2、bcl-xL. Semiquantitative analysis method based on gel electrophoresis image was adopted to compare gene expression among groups. RESULTS : The gene expression of bcl-2、bcl-xL significantly increased in GBE50 group compared with control group. CONCLUSION: The novel Ginko biloba extract GBE50 can promote expression of the antiapoptotic gene bcl-2、bcl-xL in rabbit myocardium.