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黄瓜SRAP遗传连锁图的构建及始花节位的基因定位 被引量:129

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摘要 在由黄瓜自交系S06与S52杂交产生的F2群体中,应用SRAP(sequence-related ampli-fied polymorphism)标记构建黄瓜的分子遗传连锁图谱.使用了64个多态性引物组合,共得到108个多态性条带,单对引物组合最多的产生5个多态性条带,平均1.7个.在F2群体中对这些多态性位点进行连锁分析,并用Mapmaker 3.0构建连锁群,77个标记位点进入9个连锁群(LOD≥3.0),总长1114.2 cM,标记平均间距14.5 cM,标记均匀分布于整个连锁群.同时将始花节位性状控制基因ffn(first flower node)定位在第Ⅸ连锁群上,与两侧标记DC1EM5和ME7EM2A的距离分别为10.3和12.1 cM.
出处 《自然科学进展》 北大核心 2005年第2期167-172,共6页
基金 上海市科委(批准号:033107019)上海市农业委员会(批准号:沪农科攻字(2003)第2-1-2号)资助项目
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