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微小牛蜱铁蛋白编码基因的克隆和分析 被引量:11

Molecular Cloning and Analysis of Ferritin of Boophilus Microplus
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摘要 从微小牛蜱克隆到1个新的铁蛋白编码基因,cDNA全长642bp,编码区为123 639bp,编码172个氨基酸残基,该蛋白预测的分子量为19 9ku,等电点为4 24。经过分析,其预测氨基酸序列与已报道的变异革蜱、非洲钝缘蜱和蓖子硬蜱铁蛋白同源性分别为93 60%、88 37%和83 72%。且核苷酸序列在mRNA5′未翻译区(5′UTR)的茎环结构存在铁应答元件(IRE),其氨基酸序列上带有典型的亚铁氧化酶中心结构的保守序列。RT PCR分析表明,该基因在微小牛蜱卵、幼蜱、半饱血雌蜱、饱血雌蜱和雄蜱这几个阶段均有表达。 A gene encoding ferritin was isolated and cloned from B. microplus. The cDNA is composed of 642 nucleotides and encoding 172 amino-acid residues, the encoding region is between 123bp to 639bp, the deduced mass is 19.9ku and isoelectric point is 4.24.The deduced amino-acid sequence shows 93.60%, 88.37% and 83.72% similarities with the ferritin from tick Dermacentor variabilis, Ixoded ricinus, and Ornithodoros moubata respectively. The stem-loop structure of a putative iron responsive element is found in the 5-untranslated region of ferritin mRNA. The protein contains the conserved motifs for ferroxidase center. RT-PCR analysis suggests the ferritins are expressed in the eggs,larvas,fed female adult,engorgement female adult and male adult ticks of B. microplus.
出处 《畜牧兽医学报》 CAS CSCD 北大核心 2005年第1期66-69,共4页 ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金 科技部基础研究重大项目前期研究专项(2001CCA00900)
关键词 铁蛋白 编码基因 微小牛蜱 克隆 表达 编码区 氨基酸序列 硬蜱 保守序列 RT-PCR分析 Boophilus microplus ferritin cDNA cloning RT-PCR
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