摘要
4只杂种奶山羊按5g/kg体重剂量投服披针叶黄华干草粉,分别于试验的第0、7、14、28、35、42天采血样作血常规、血清酶学及血清生化指标检验,并每天观察临床症状,试验结束时剖检进行病理学检查,结果表明:受试羊于试验的第30天开始出现中毒症状,表现为体重下降,机体消瘦,被毛粗乱,于试验的第41天出现运动异常,驱赶时突然倒地;血红蛋白含量和白细胞数升高,与试验前比较差异显著(P<0 05);血清谷草转氨酶活性和碱性磷酸酶活性明显升高,与试验前相比较差异极显著(P<0 01);血清尿素氮从第14天起显著下降,与试验前比较差异极显著(P<0 01);剖检变化以组织器官肿大、出血为主,组织学检查以组织脏器出现空泡化为主,超微结构检查,首次观察到肝脏胶原纤维增生。
Four crossed-breed dairy goats were fed on Thermopsis Lanceolat R.br dry hay powder at dosage of 5 g/kg body weight,blood samples were collected on the 0th, 7th, 14th, 28th, 35th and 42nd experimental day for examining routine blood indexes, serology enzymology and biochemical indexes. The clinical symptom of the dairy goats was observed every day and the goats were dissected to conduct pathological examination in the end of the experiment. Results showed that the toxic symptom of tested dairy goats occurred in the 30th experimental day with decreased weight, thin body, and coarse fur. In the 41st experimental day, the tested dairy goats began to move abnormally, and suddenly fell down to the ground when being driven away. In contrast to undosed period, the content of hemoglobin and the number of white cells of the tested dairy goats increased significantly (P<0.05) and activity of glutamic-oxalacetic transaminase (GTP) and alkaline phosphatase (APH) increased significantly (P<0.01). Urea nitrogen level in serum decreased significantly (P<0.01) from the 14th experimental day.Pathological changes mainly showed tissue organism was turgescent and bleeding. Histological examination mainly showed vacuolar degeneration in the tissue organism. It was the first time to observe collagenous fiber hyperplasy in liver by ultra structural examination.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2005年第1期82-86,共5页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金(39770571)
