亚低温缺氧对大鼠脑单胺类递质和血清电解质含量的影响

The effects of subhypothermia and hypoxia on the contents of brain monoamine neurotransmitters and serum electrolyte

目的研究亚低温低压缺氧对大鼠脑单胺类神经递质含量和血清电解质水平的影响。方法采用48只健康成熟雄性Wistar大鼠，分别在10℃和20℃环境条件下，地面和模拟上升至3．0km和5．0km高度停留lh，然后以30m／s速度降至地面，立即取大鼠脑组织制成匀浆测定5-羟色胺(5-HT)、去甲肾上腺素(NE)和多巴胺(DA)的含量；取大鼠血清检测钠(Na^+)、钾(K^+)和钙(Ca^2+)含量的变化。结栗在10℃环境5．0km组的血清Na^+、K^+及脑组织匀浆中5-HT含量均低于相应的地面组(P＜0．05或P＜0．01)，但脑组织匀浆NE含量显著升高(P＜0．05)；在10、20℃环境中3．0km组脑组织匀浆5-HT含量均低于相应的地面组(P＜0．05)。结论亚低温环境中，高空缺氧可引起血清Na^+、脑组织5-HT含量降低，NE含量升高。

Objective To study the effect of subhypothermia and hypoxia on the contents of brain monoamine neurotransmitters and serum electrolyte.Methods Respectively under the condition of 10℃ and 20℃,48 healthy,male adult Wistar rats were dealt with on the ground,then raised by imitation to a height of 3.0 km and 5.0 km staying for one hour,later descended to the ground at a speed of 30 m/s,and after that the brain tissue was immediately taken for preparation of homogenate for content detection of 5 HT NE and DA...