降解对苯二甲酸厌氧微生物群快速驯化与富集技术(英文)

Quick Enrichment & Acclimation of an Anaerobic Microbial Consortia Used for Biodegrading Teraphthalic Acid (TA)

以 TA为唯一碳源 ,在反硝化条件下对取自甲烷发酵反应器中的厌氧污泥进行驯化 ,最初的 3周内 ,保持反应器中 KNO3的浓度在 5.0 g/ L,第四周降到3 .0 g/ L,第五周降到 0 .4 g/ L,第六周起不添加 KNO3再经过 5周时间驯化 .经过约 90天的驯化 ,原存在于种污泥中的发酵性细菌几乎全部消亡 ,新的培养物中取而代之的是 TA还原和开环菌 .

The seed sludge originated from a methane fermentation reactor was enriched and acclimated with TA as sole carbon source under nitrate respiration mode first, with 5.0g/L KNO 3 being added in the first three weeks, 3.0g/L in the 4th, 0.4g/L in the 5th, and zero addition from the 6th and through another five weeks. After a total of 90 days for the enrichment and acclimation, the fermentative bacteria which originally existed in the seed sludge nearly disappeared, and instead of them, the TA reductive and cleaving bacteria group was formed in the new consortia, which was confirmed by the MPN counts and roll tube counts.