薄层扫描法测定喉舒宁片中脱水穿心莲内酯的含量

TLC scanning for the determination of dehydroandrographolide in Houshuning tablet

目的采用双波长薄层扫描法测定喉舒宁片中脱水穿心莲内酯的含量。方法薄层板为硅胶GF2 54薄层板 ,以 ψ(氯仿∶无水乙醇 ) =19∶1为展开剂 ,以 2 6 3nm为测定波长 ,370nm为参比波长。结果脱水穿心莲内酯在 1.0 0 1～ 5 .0 0 5 μg范围内浓度与峰面积线性关系良好 ,r=0 .9991,平均回收率为 10 1.0 % (RSD =2 .0 % )。

Objective To determine Dehydroandrographolide in Houshuning Tablet by TLCS. Methods Dehydroandrographolide in Herba Andrographis was determined by TLCS,ψ(Chloroform∶Ethanol,Absolute)=19∶1was used as development. Results The linear relationship was at the range of 1.001～5.005 μg(r=0.9991).The average recovery was 101.0%(RSD=2.0%). Conclusion The methods can control the quality of this preparation effectively.