有氧运动和罗格列酮对KM小鼠PPARγ、GLUT4基因表达的影响

Effects of Aerobic Exercise and Rosiglitazone on the Expression of PPARY and GLUT4 in KM Mice

为探讨有氧运动和罗格列酮调节体内糖代谢的作用及机制,将KM小鼠分为3组:对照组(Ⅰ)、用药组(Ⅱ)和用药运动组(Ⅲ)。观察体重和脂肪的变化;RT-PCR半定量肝脏、脂肪和肌肉组织PPARγ利GULT4mRNA表达。结果显示:1.Ⅱ利Ⅲ组的体重和脂肪重量显著人于Ⅰ组,Ⅲ组与Ⅱ组相比体重和脂肪重量有所下降:2与Ⅰ组相比较,Ⅱ和Ⅲ组肝脏、肌肉和脂肪组织的PPARγ表达增加1-2倍;3.与Ⅰ组相比较,Ⅱ和Ⅲ组脂肪和骨骼肌GULT4表达均增加1倍以上。提示:(1)罗格列酮引起的增肥现象可通过适当运动来控制。(2)罗格列酮和有氧运动可提高肌肉和脂肪组织的GLUT4的表达,可能由PPARγ介导调控GLUT4转录。

To expolre the regulating action to glucose metabolism and their mechanism of aerobic exercise and Rosiglitazone, the KM mice were divided into three groups: Controlled group (group Ⅰ); Treated with rosigiitazone for 8 weeks (group Ⅱ); Treated with rosigiitazone + aerobic running at speed 14m/min for 1hr/d for 8 weeks (group Ⅲ). Body weight and belly fat weight were measured; The mRNA expression of PPARy and GULT7 were determined by semi-quantitative RT - PCR. Results: 1. Rosiglitazone caused a significant increase in body weight and fat weight in Ⅱ and Ⅲ groups compared with groupⅠ; groupⅢ's body weint and fat decreased corpared with group Ⅱ. 2. The mRNA expession of PPARr was increased 1-2 folds in Ⅱand Ⅲ groups companed with group Ⅰ in the three tissues respectively. 3. The level of GLUT4 mRNA of Ⅱ and Ⅲ groups was over 1 -fold higher than that of group I in muscle and adipose tissue. The results indicated: 1. The effect of fat-increased induced by rosigiitazone can be reduced through aerobic exercise. 2. Rosiglitazone and aerobic exercise enhanced the express of GLUT4 in muscle and adipose tissue. It is possible that PPARs medied GLUT4 transcripting.