贵州牛带绦虫亚洲亚种实验感染家猪的研究

EXPERIMENTAL INFECTION OF DOMESTIC PIGS BY TAENIA ASIATICA

目的 观察贵州省牛带绦虫亚洲亚种囊尾蚴在猪体内的发育情况。 方法 用牛带绦虫亚洲亚种孕节直接灌喂当地圈养的 14d龄健康乳猪 8头 ,并以健康乳猪 4头作对照。于感染后 40、5 0、60和 75d分别剖检 ,观察猪体内囊尾蚴的分布、大小、形态及成熟情况。 结果 从感染后 40d起 ,在 6头猪的肝脏表面和实质内共检获 42 1个囊尾蚴 ,多分布在肝实质内 ,其大小为 1.3 4～ 2 .78mm× 1.2 2～ 2 .3 1mm。其余部位未找到囊尾蚴。感染后 40d和 5 0d ,囊尾蚴经胆汁孵育 ,头节翻出率分别为 78.3 %和 93 .4%。感染后 5 0d开始发现钙化的囊尾蚴。囊尾蚴活体观察可见头节上 4个吸盘和活动的顶突。压片染色镜检 ,头节有 4个吸盘和顶突 ,顶突周围发现不完整的两圈点状结构。 结论 根据囊尾蚴的分布、大小、发育时间及形态特征 ,进一步认定贵州省都匀市的带绦虫为牛带绦虫亚洲亚种。家猪是其可能的中间宿主。

Objective To observe the development of cysticercus of Taenia asiatica in the body of domestic pigs. W Methods The experimental animals were 8 Yoek pigs who were 14 days old, every pig was fed with 3 gravid proglottids of T. asiatica . These pigs were dissected at 40 d, 50 d, 60 d and 75 d after infection respectively, and the distribution, size, conformation and development of cysticercus in the body of pigs were observed. W Results 6 of 8 experimental pigs were infected by cysticercus. Total of 421 cysticerci was found on the surface(187) and in the parenchyma(234) of liver in 6 pigs. The size of cysticercus were 1.34-2.78 mm× 1.22 -2.31 mm. When cysticerci after infection 40 d were cultured in 40% gall, 78.3%(18/23) cysticerci stretched their scolex, but 93.4%(57/61) cysticerci could do in cysticerci after infection 50 d. Calcified cysticerci were found after infection 50 d. There were 4 suckers and a moveable rostellum on the scolex, moreover, two cycles of granular structure were found in seven scolex. W Conclusion The tapeworm endemic in Guizhou was further confirmed to be T. asiatica . Domestic pigs may be a suitable intermediate host of T. asiatica .