流动注射化学发光法测定头孢氨苄和头孢拉定

Flow-injection chemiluminescence determination of cephalexin and cephradine in pharmaceuticals

基于KMnO4在酸性介质中氧化头孢氨苄和头孢拉定产生微弱的化学发光,同时借助甲醛对发光的增强作用,采用流动注射技术,建立了以上两种头孢类抗生素药物的测定新方法。方法检出限分别为0.096和0.10μg mL;相对标准偏差分别为1.6%和1.8%(n=11),线性范围分别为0.8～25和0.8～32μg mL,进样频率为60次 h。本方法已用于药物制剂的测定。

A new chemiluminescencence method for the determination of cephalexin and cephradine with flow injection technique has been developed. The mothod is based on the enhancement of formaldehyde on the chemiluminescence reaction between KMnO4 and the above cephalosporins. The detection limit is (0.096 μg/mL) for CPX and 0.10 μg/mL for CPD with corresponding relative standard deviation of 1.8% and 2.0%(n=11). The linear range is 0.8～(25 μg/mL) for CPX and 0.8～32 μg/mL for CPD. The sampling frequency is 60 times per hour. The method is easy and rapid and has been applied to the determination of CPX and CPD in drugs successfully.