胰岛素对全血血小板膜P-选择素表达影响的研究

Effect of insulin on expression of whole-blood platelet membrane P-selectin

目的探讨胰岛素对血小板膜P-选择素表达的影响。方法建议全血血小板膜P-选择素表达的流式细胞术(FCM)测定方法,观察健康青年人胰岛素在静止和活化状态下对血小板膜P-选择素表达的影响。结果胰岛素对正常人静息血小板膜P-选择素表达无明显影响;而显著抑制凝血酶、胶原活化的血小板膜P-选择素的上调表达,且该效应呈剂量和时间依赖性。结论胰岛素可抑制活化血小板膜P-选择素的表达。

Objective To investigate the effects of insulin on the expression of whole-blood platelet membrane P-slectin. Methods Flow cytometry (FCM) was used to detect the expression of whole-blood platelet membrane P-slectin, and the effect of insulin on the expression on quiescent and activated plasma platelet observed in normal young subjects. Results Insulin had no effect on the P-slectin expression on quiescent platelet membrane, but significantly inhibited the up-regulation of P-slectin expression induced by thrombin and collagen respectively, exhibiting dose- and time-dependent effects. Conclusion Insulin can inhibit P-slectin expression on activated platelet membrane.