布比卡因对Balb/c和C57BL/6小鼠胃酸分泌功能的影响

Effects of bupivacaine on the gastric acid secretion in Balb/c and C57BL/6 mouse

目的 探讨布比卡因对Balb／c:和C57BL／6小鼠胃酸分泌功能的影响。方法 应用幽门结扎术测定了基础状态下和布比卡因局部镇痛后3小时Balb／c和C57BL／6小鼠的胃酸量(ml),胃酸度(mEq／L)和总酸量(μEq／3h),并将基础状态下两种小鼠和各自应用布比卡因前后的胃酸分泌指标进行比较。结果 基础状态下Balb／c小鼠(n=9)的胃酸量和总胃酸量显著高于C57BL／6小鼠(n=10)的胃酸量和总胃酸量(分别为2.319±0.181和0.825±0.062,P<0.001;29.105±4.90和8.565±1.203,P<0.001);Balb／c小鼠的胃酸度稍高于C57BL／6小鼠(分别为12.311±1.737和10.36±1.313,P=0.37),但无统计学差异。应用布比卡因局部麻醉后Balb／e小鼠(n=6)的胃酸量和总胃酸量显著低于基础状态下的分泌量(分别为2.319±0.181和1.529±0.142,P<0.05:29.105±4.90和11.956±2.516,P<0.05);应用布比卡因局部麻醉后Balb／c小鼠的胃酸度低于其基础状态下的胃酸度(分别为12.311±1.737和6.433±1.189,P=0.07)。应用布比卡因局部麻醉后C57BL6小鼠(n=6)胃酸量、酸度和总胃酸量和基础状态下的分泌量无显著差异(分别为0.825±0.062和0.863±0.105,P=0.84;8.565±1.203和8.725±1.102,P:0.95;10.36±1.313和12.433±1.27,P=0.46)。结论 基础状态下Balb／e利C57BL／

Objective To investigate the effects of Bupivacaine on the gastric acid secretion in Balb/c and C57BL/6 mouse .Methods Measuring 3 h gastric acid volume (ml),acidity (mEq/L) and total acid output (μEq/3 h) in pylorus-lig ated Balb/c and C57BL/6 mouse in basal state and after localized use of bupivacaine. Results Gastric acid volume and total acid output were significantly higher in Balb/c (n =9) than those in C57BL/6 (n = 10) (2 .319 ± 0.181 vs 0.825 ± 0.062, P< 0.001; 29.105±4.90 vs8.5654± 1.203, P = 0.0005 respectively); Acidity was slightly higher in Balb/c than that in C57BL/6 (12 .311 ± 1 .737 vs 10.364±1.313 respectively, P = 0.37). Gastric acid volume and total acid output after localized use of Bupivacaine (n = 6) were markedly lower than those in basal state (2 .3194 ±0.181 vs 1.529 ± 0.142, P = 0.036; 29.1054 ± 4.90 vs 11 .9564 ±2.516, P = 0.04 respectively) in Balb/c mouse; Acidity after localized use of Bupivacaine was lower than that in basal state (12.311± 1.737 vs6.433± 1.189, P = 0.07) in Balb/c mouse. There were no significant differences on gastric acid volume, acidity and total acid output before and after localized use of Bupivacaine ( n = 6) (0.825 ±0.062 vs 0.863±0.105, P = 0.84; 8.565 ±1.203 vs 8.725 ± 1.102, P = 0.95; 10.36 ± 1.313 vs 12.433 ± 1 .27, P = 0.46 respectively) . Conclusion There is a significant difference on gastric acid secretion between Balb/c and C57BL/6 mouse in basal state. Bupivacaine markedly decrease gastric acid volume and total acid output but has weaker effect on acidity in Balb/c mouse and has no inhibitory effect on gastric acid secretion in C57BL/6 mouse.