E1B缺陷腺病毒dl1520联合化疗药物DDP体外对鼻咽癌细胞杀伤及诱导凋亡作用

The effect of E1B-deleted adenovirus, dl1520 with DDP on NPC cell killing and apoptosis in vitro

目的探讨E1B-55KDa缺陷腺病毒dl1520与DDP联合体外对鼻咽癌细胞的杀伤及诱导凋亡作用。方法采用MTT法体外测定细胞生长抑制率,流式细胞仪检测细胞凋亡。结果MOI(感染复数)=0.032、0.16、0.8、4、20、100时dl1520对CNE-2细胞的生长抑制率分别为2.78%、7.41%、10.19%、24.07%、45.37%、67.59%,而dl1520+DDP的抑制率分别为29.63%、32.41%、40.74%、52.78%、66.67%、74.07%,P<0.01;1.5μg/mlDDP24h诱导CNE-2细胞凋亡的比例为1.7%,dl1520在MOI=0.1、1、10、100时诱导的凋亡比例分别为1.2%、1.3%、14.1%、2.7%,而当dl1520与DDP联合后诱导的凋亡比例分别为1.8%、3%、18%、3.2%。结论dl1520与DDP联合可显著增强DDP对CNE-2细胞的杀伤及诱导细胞凋亡作用。

Objective To evaluate the anti-NPC effect of E1B-55Kda deleted adenovirus dl1520 with DDP in vitro. Methods Detecting the cytotoxicity by MTT assay; apoptosis cells detected by flow cytometry. Results In MTT assay, the inhibitory rate of cell viability in dl1520 group were 2.78%, 7.41%, 10.19%, 24.07%, 45.37%, 67.59% when MOI(multiplicity of infection)were 0, 0.032, 0.16, 0.8, 4, 20, 100 respectively; But in dl1520+DDP group,the inhibitory rate was 24.07%, 29.63%, 32.41%, 40.74%, 52.78%, 66.67%, 74.07% respectively, P<0.01. When MOI were 0, 0.1, 1, 10, 100,the percent age of apoptosis cells was 1.2%, 1.2%, 1.3%, 14.1%, 2.7% respectively. After plus DDP,the percent age of apoptosis cells was 1.7%, 1.8%, 3%, 18%, 3.2% respectively . Conclusion dl1520 with DDP may significantly potentiate the cell-killing and apoptosis on NPC cell.