广东地区汉族正常人群HLAE基因多态性研究

Analysis on HLA E Polymorphism in Guangdong Han Population

为了检测广东地区汉族正常人群HLA E基因的多态性 ,并分析HLA E与典型HLA I类 (Ia)位点之间的连锁关系 ,采用PCR SSP方法检测广东地区 15 0个无血缘相关的正常人的HLA E基因型 ,同时用NIH标准微量淋巴细胞毒方法检测其中 10 6人HLA A , B基因型 ,对HLA E与HLA A , B位点之间作连锁分析。结果发现 ,在广东地区汉族正常人群中共检出 3个HLA E等位基因 :HLA E 0 10 1,HLA E 0 10 3 1,HLA E 0 10 3 2 ,其基因频率分别为 4 5 .3 3 % ,3 2 .3 3 % ,2 2 .3 3 %。未检出E 0 10 2和E 0 10 4。HLA E与HLA A , B位点之间B15 / E0 10 3 2及A2 /E 0 10 3 2存在连锁不平衡 ,除此之外其他任何两位点之间不存在连锁不平衡。结论 :HLA E高度保守的多态性提示其生物学特性可能有别于HLA Ia分子。

To investigate the distribution of HLA E alleles and linkage between HLA E and HLA A or B loci in Chinese Han in Guangdong area, HLA E alleles were detected by using PCR SSP in 150 unrelated healthy individuals from Guangzhou area; HLA A, B antigens typing in 106 individuals was carried out with NIH standard microlymphocytoxic method. Analysis of linkage was performed between HLA E and HLA A, B. The results showed that three alleles of HLA E could be detected in this population. They are E*0101,E*01031,E*01032, with the frequency of 45.33%,32.33%,22.33% respectively. No E*0102 and E*0104 could be detected in all of these individuals. The analysis of linkage on two loci between HLA E and HLA A or B showed that no significant difference could be found between expected frequencies and observed frequencies except B15/E*01032 and A2/E*01032. In conclusion, the high conservative polymorphism of HLA E suggests that it′s biological characteristic is different from that of classical HLA class Ia molecules.