涅斯捷连科氏菌属菌株的分离及系统学研究

Study on Isolation and Systematic Taxonomy of Strains of Genus Nesterenkonia

从我国新疆及埃及东部高盐环境中分离到 4株形态上类似于涅斯捷连科氏菌属的革兰氏阳性菌株。以该属的 2个典型菌株 (NesterenkoniahalobiaDSM 2 0 5 4 1T和NesterenkonialacusekhoensisDSM 12 5 4 4 T)为对照 ,对 4个分离菌株进行了包括形态学 ,生长pH范围 ,温度范围 ,耐NaCl,KCl,MgCl2 ·6H2 O ,CaCl2 实验及酶学特性以及细胞化学组份、(G +C)mol%含量测定 ,16SrDNA分析及DNA DNA杂交在内的多相分类研究。结果显示 ,4个分离菌株属于涅斯捷连科氏菌属 ,但与涅斯捷连科氏菌属的 2个有效发表种有显著差异 ,因此

Four Gram-positive, Nesterenkonia-like strains were isolated from hypersaline soils collected from Xinjiang Province, China and East of Egypt. The four strains were studies using a polyphasic approach, which included morphological observation, growth pH and temperature rang, tolerance to NaCl,KCl,MgCl 2·6H 2O,CaCl 2, enzyme profiles, chemotaxonomy, (G+C) mol% content, 16S rDNA sequences analysis and DNA-DNA hybridization, and the only two type strains of the genus Nesterenkonia, Nesterenkonia halobia DSM 20541 T and Nesterenkonia lacusekhoensis DSM 12544 T were used as control. The test results showed the four isolates belonged the genus Nesterenkonia, but they had great differences from the other two valid species of the genus Nesterenkonia, and thus they should represent four new species of this genus.