成都汉族群体六个短串联重复序列基因频率及其种属特异性研究

Allele frequencies and species specificity of six short tandem repeat loci in Chinese population

目的对成都汉族群体6个短串联重复序列等位基因频率及其种属特异性进行研究,探讨其在法医学中的应用价值。方法用PCR扩增、非变性聚丙烯酰胺凝胶电泳、硝酸银染色技术,对110名成都汉族无血缘关系健康个体的6个短串联重复序列等位基因频率及其种属特异性进行研究。结果D4S2366、D4S2367、D6S474、D6S1281、D2S1396和D20S601在成都汉族群体中等位基因个数分别为7、7、6、7、5、7;观察杂合度分别为0.802、0.708、0.770、0.627、0.542、0.672;个人识别率分别为0.887、0.828、0.849、0.848、0.794、0.865;非父排除率0.602、0.441、0.544、0.325、0.227、0.386。通过种属特异性的评价发现6个短串联重复序列与其他种属间存在较大差异。结论6个短串联重复序列在成都汉族群体中具有较好的遗传多态性及种属特异性,在法医学研究中具有应用价值。

Objective To develop a set of new markers for forensic application, the authors have chosen 6 short tandem repeat(STR) loci to study the allele frequencies and species specificity in Chinese Han population in Chengdu. Methods One hundred and ten EDTA blood samples were collected from the unrelated individuals in Chengdu city, Sichuan province. DNA was extracted by Chelex 100 and amplified by the polymerase chain reaction(PCR). Polyacrylamide gel electrophoresis (PAGE) and silver staining were used to analyze the PCR products. Results The polymorphisms of all 6 STR loci have been obtained in Chinese Han population in Chengdu, the alleles of D4S2366,D4S2367,D6S474,D6S1281,D2S1396 and D20S601 being 7,7,6,7,5,7, the observed heterozygosity of them being 0.802,0.708,0.770,0.627,0 542,0.672, the discrimination power of them being 0.887,0.828,0.849,0.848,0 794,0.865; and the power of exclusion of them being 0.602,0.441,0.544,0.325,0.227,0.386. Evaluated by comparison with the data from 14 different animals as controls, the 6 STR loci contain good specificity of human beings. Conclusion The 6 STR loci are highly polymorphic and can play a key role in species identification. They are new candidate markers for forensic personal identification and paternity testing.