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蛋白激酶C激活调控Bcl-2与缺血诱导神经元凋亡关系的研究 被引量：1

Study of Relationship between Bcl-2 Regulated by Activated Protein Kinade C and Neuronal Apoptosis Induced by Ischemia
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摘要 目的:探讨蛋白激酶C激活参与神经元凋亡的可能机理。方法:采用大鼠全脑缺血模型,观察脑缺血／再灌流后蛋白激酶C活性、Bcl-2表达及神经元凋亡的变化。结果:脑缺血／再灌流可以导致蛋白激酶C的移位激活伴Bcl-2表达及神经元凋亡的增加;用蛋白激酶C抑制剂灯盏花可以阻止上述变化。结论:蛋白激酶C的激活促进Bcl-2表达可能与脑缺血／再灌流诱导的神经元凋亡有关。 Objective To explore the mechanism of protein kinase C (PKC) involved in neuronal apoptosis induced by cerebral ischemia/reperfusion. Methods After the model of ischemia/reperfusion was established in male Wislar rats, the changes in PKC activity, Bcl-2 protein expression and neuronal apoptosis were determined. The effect of PKC inhibitor (Denyzhanghua injection) on PKC activity,Bcl-2 protein expression and neuronal apoptosis was observed. Result The cerebral ischemia/reperfusion could result in PKC translocational activation accompanied with the increases in the experssion of Bcl-2 protein and neuronal apoptosis. Denyzhanghua could prevent the above-mentioned changes. Conclusion The increase in the expression of Bcl-2 produced by activated PKC may be related to the neuronal apoplosis induced by the cerebral ischemia/reperfusion.
作者 陈康宁 李云峰
机构地区 第三军医大学第一附属医院神经内科 济南军区总医院神经内科
出处 《华南国防医学杂志》 CAS 2004年第4期25-28,共4页 Military Medical Journal of South China
基金 国家自然科学基金项目(编号39670269)
关键词 神经元凋亡 蛋白激酶C 再灌流 BCL-2表达 激活 脑缺血 诱导 研究 活性 机理 Neuronal apoptosis Protein kinase C Bcl-2 protein Cerebral ischemia/reperfusion
分类号 R743 [医药卫生—神经病学与精神病学] R735 [医药卫生—肿瘤]
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参考文献11

  • 1[1]Pongracxz K,Deacpm EM,Johson GD,et al .Doppa induces cell death but not differentiaation of U 937 cells: evidence for the involvement of PKC β1 in the regulation of apoptosis .leak Res,1996,40(4) :319～326
  • 2[2]Pulsinelli WA,Brierley J B.A new model of bilateral in the unanesthesized rat.hemispheric ischemia.Stroke,1979,10(3) :267～272
  • 3[3]Crumrine RC,Dubyak G,LaManna JC.Decreased protein kinase C activity during cerebral ischemia and after reperfusion in adult rat.J Neurochem,1990,55(6) :2001～2007
  • 4[4]Li Y,Chopp M,Garcia JH,et al .Induction of DNA fragmentation after 10 - 12) minutes of focal cerebral ischemia in rats.Stroke,1995,26(7): 1292～1298
  • 5[5]Domaska JK,Domanka- janik K,Zablocka B.Protein kinase C as a early and sensitive maker of ischemia - induced progressiveneuronal damage in gerbil hippocampus.Mol Chem Neuropathol,1993,20(2):111～123
  • 6[6]Nishizuka Y.Intracellullar signaling by hydrolysis of phospholipid and activation of protein kinase C.Science 1992,256:607～614
  • 7[8]Heron A,Pollard H,Dessi F,et al .Regional variability in DNA fragmentation after global ischemia evidenced by combined histological and gel electrophoresis obeserbations in the rat brain.J Neurochem,1993,61(5): 1973～1976
  • 8[9]Goto K,Ishige A,Sekiguchi K,etal .Effects of cycloheximide on delayed neuronal death in rat hiplocampus.Brain Res,1990,534(1～2):299～302
  • 9陈康宁,董为伟,张帆.蛋白激酶C激动剂PMA对培养神经元胞内游离钙的影响[J].第三军医大学学报,1997,19(5):408-410. 被引量：18
  • 10[11]MacManas JP,Hill IE ,Preston E,et al .Difference in DNA fragmentation following Tranient cerebral or decapitation ischemia in rat .J Cereb Blood Flow Metab,1995,15(5) :728～737

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同被引文献7

  • 1D'Cruz B J, Fertig K C, Filiano A J, et al. Hypothermic reperfusion after cardiac arrest augments brain-derived neurotrophic factor activation [ J ]. J Cereb Blood Flow Metab, 2002,22 (7) : 843-851.
  • 2Verkman A S, Binder D K, Bloch O, et al. Three distinct roles of aquaporin-4 in brain function revealed by knockout mice [J]. Biochim Biophys Acta, 2006,1758(8) : 1085-1093.
  • 3Niwa M, Inao S, Takayasu M, et al. Time course of expression of three nitric oxide synthase isoforms after transient middle cerebral artery occlusion in rats [J]. Neurol Med Chir (Tokyo), 2001,41 (2) :63-72.
  • 4White B C, Sullivan J M, DeGracia D J, et al. Brain ischemia and reperfusion: molecular mechanisms of neuronal injury [J]. J Neurol Sci, 2000, 179(S1-2) : 1-33.
  • 5Zhu D Y, Deng Q, Yao H H, et al. Inducible nitric oxide synthase expression in the ischemic core and penumbra after transient focal cerebral ischemia in mice [J]. Life Sci, 2002,71 (17) : 1985-1996.
  • 6Ruan Y W, Ling G Y, Zhang J L, et al. Apoptosis in the adult striatum after transient forebrain ischemia and the effects of ischemic severity [ J ]. Brain Res, 2003,982 (2) : 228 -240.
  • 7Yenari M A, Iwayama S, Cheng D, et al. Mild hypothermia attenuates cytochrome c release but does not alter Bcl-2 expression or caspase activation after experimental stroke [J]. J Cereb Blood Flow Metab, 2002,22( 1 ) : 29-38.

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华南国防医学杂志
2004年 第4期

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