芍萸复方对血虚小鼠芍药苷血药浓度的影响

Effects of Shaoyu decoction (芍萸复方) on paeoniflorin concentration in plasma of blood deficiency mice

目的 :比较健康和血虚模型小鼠口服芍萸复方后血中芍药苷浓度 ,探讨“血虚证”与白芍效应成分芍药苷药代动力学的关系。方法 :失血法制备血虚模型 ,高效液相色谱技术检测小鼠灌胃 1小时后血中芍药苷的浓度。结果 :芍药苷与血浆中其它成分能很好分离 ,在 5 .0～ 2 5 0 .0ng/μl范围内线性关系良好 ,平均回收率为 95 .5 2 % ,最低检测浓度 1.49ng/μl。正常对照组小鼠平均血药浓度为 63 .8± 2 .45ng/μl,血虚模型组为 117.84± 6.3 5ng/μl。 结论 :血虚证鼠血中芍药苷浓度较正常小鼠高。

Objective: To observe the effect on plasma concentration of paeoniflorin in healthy and blood deficiency model mice after taking Shaoyu decoction. Methods: The determination was carried on RP HPLC. The conditions of chromatography:stationary phase: A C18 radial compression column(250mm×4.6mm,7um) , mobile phase: CH 3OH H 2O (38:62), flow rate: 0.5ml/min, detection wavelength: 230nm. To compare plasma concentration of paeoniflorin in control group (healthy mice) with treat group (blood deficiency mice) after 1 hour ig. Results The linear range of paeoniflorin was 5.0～250.0ng/μl, the limit of detectability was 1.49ng/μl, the average recovery was 95.52%. The concentration of paeoniflorin in plasma for control group was 63.8±2.45ng/μl, treat group was 117.84±6.35ng/μl. There was significant difference between two groups (p<0.001). Conclusion: The concentration of paeoniflorin in plasma of healthy mice was lower than blood deficiency mice.