多光子近场共焦光学扫描显微镜表面非传播光场的特性研究

Trait researches on no propagation light field multi-photon near-field confocal optical scanning mocroscopy

对多光子近场共焦光学扫描显微镜非传播光场的特性进行了系统的理论分析 ,导出了多光子近场共焦光学扫描显微镜表面非传播光场的频率宽与相差的测不准关系表达式及二维的点扩散函数光学传递函数和能量分布表达式。研究结果表明 :多光子近场共焦光学扫描显微镜比单光子共焦显微镜具有更高的横向分辨率和纵向分辨率 ,而多光子近场共焦光学扫描显微镜比双光子共焦显微镜具有更高的空间分辨率 ,样品置于其表面的非传播光场内 ,能得最佳的超分辨效果。

The Imaging theory on no propagation light field of multi photon near field confocal optical scanning mocroscopy is presented.The frequency width and time gap indefinite determining the two dimensional point sprend function.two dimensional optical transfer function and energy coupling on no propagation light field of multi photon near field confocal optical scanning mocroscopy is derived.By using those theory,We have proved that the horizontal and vertical resolutions of two photon confocal scanning microscopy are higher than those of the single photon confocal scanning microscopy,and the space resolution of multi photon confocal scanning microscopy is higher than that of two photon confocal scanning microscopy and the space resolution of article by put in no propagation light field is best.