侧脑室内微量注射毒扁豆碱对大鼠胃运动的影响

Effect of Intracerebroventricular Microinjection of Physostigmine on Gastric Motility in Rats

用定点记录胃运动法,观察脑室注射(icv)毒扁豆碱(PHY)对大鼠胃运动的影响。icv PHY可使胃运动出现增频期、增幅(张力)期、抑制期和恢复期,预先icv阿托品或切断双侧膈下迷走神经可完全阻断以上效应;将动物利血平化或icv心得安均明显减弱胃张力并取消抑制相;icv哌唑嗪或育亨宾对其无影响。提示:中枢内源性乙酰胆碱增强胃运动频率和张力是通过M受体并经迷走神经传出实现的;抑制相可能与体内儿茶酚胺类和中枢β受体有极密切的关系。

Using the fix-points method for recording gastric motility, the effects of intracerebroventricular (icv) microinjection of physostigmine (PHY) were observed in Wistar rats. The results showed that the gastric motility displayed an increased frequency phase, an increased amplitude phase, a phase of inhibition and a phase of restitution after icv injection of PHY. These effect were blocked completely either by pretreatment of atropine, or by vagotomy. Pretreat-ment with reserpine or icv injection of propranolol remarkably impaired the gastric tension and abolished the inhibition phase; while icv injection of prazocine or yohimbine was of no effect.These results suggest that enhancement of gastric motility elicited by endogenous acetylcholine in central nervous system seemsto be achieved through cholinergic muscarinic receptors and via vagus nerves and that the phase of inhibition in the process of gastric motility might beclosely related to adrenergic β-receptors of the central nervous system and cate-cholamine (CA) in vivo.