实验性肝癌超微结构的体视学研究

A Stereological Study of the Ultrastructure of Experimental Hepatocellular Carcinoma

应用电镜技术和体视学定量分析方法,在二甲氨基偶氮苯诱发大鼠肝癌的癌发生过程中的各个不同时期,对肝细胞核、核仁及核仁颗粒成分的体密度进行了定量分析。结果表明:在给予致癌剂后,这三种成分的体密度值均呈上升改变,至肝癌形成后即明显高于正常,其在癌细胞与非癌细胞之间的差异有非常显著性意义(P<0.01)。这对于较确切地了解癌发生过程中不同时期的细胞功能状态及确定其恶性程度有一定的帮助。

The volume density of nuclei, nucleoli and nucleolar granular components of the rat liver cells were analyzed by using electron microscopic and morphometric techniques during hepatocarcinogenesis induced by dimethyl-aminoazobenzene. The results showed that the volume density of the above-mentioned three kinds of nuclear components began to rise after administration of carcinogen and was markedly higher than that of normal cells when hepatocar-cinoma developed, and the differences between that of hepatoma cells and that of non-hepatoma cells were highly statistically significant (P<0.01). This may be helpful in understanding the functional state of cells at different stages of hepatocarcinogenesis and in defining the grade of carcinoma malignancy.