武汉地区胰岛素依赖型糖尿病病人补体型的初步检测

Investigation of Genetic Polymorphism of Complotypes in Patients with Insulin-Dependent Diabetes Mellitus in Wuhan District of China

对武汉地区22个胰岛素依赖型糖尿病(IDDM)病人家系成员的补体C2、Bf、C4A 与C4B的遗传多态性进行了检测,并统计分析了这4个成分组成的补体型分布及其频率。在病人44条染色体上共检出12种补体型,其中以SC31(0.386)与SC21(0.182)两种频率最高,与正常人频率分布接近。除3种补体型S(07)C01、S(07)C0(92)和FC20(各1例)仅见于病人外,其余7种IDDM与正常人比较也无显著差异,补体型SC32和SC22在正常人的频率分别为0.058和0.047,而在病人中未检出。此外还有 17种罕见补体型也未见于 IDDM病人中。

Tw enty two insulin-dependent diabete mellitus (IDDM) families in Wuhan district were investigated for genetic polymorphism of Bf, C2, C4A and C4B to illustrate the distribution of cbmplotypes in IDDM by family analysis. The results have been compared with those of normal families in the same district. Twelve kinds of complotypes were obtained from 44 chromosomes of IDDM patients. SC31 (0. 386) and SC21 (0. 182) are the most common complotypes in IDDM and their frequencies are similar to those found in normals (SCSI 0.378 and SC21 0.116). The next ten complotypes are SC01 (0,091), SC30 (0.091), FC21 (0.068), SC20 (0.045), FC31 (0.023), SCOO (0.023), FC2 (92) (0. 023), S (07) C01 (0. 023), S (07) CO (92) (0. 023) and FC20 (0. 023) in frequency order in IDDM. Compared with those of normals, the former seven complotypes could not show significant difference between IDDM patients and normals; the last three complotypes S (07) C01, S (07) CO (92) and FC20 could only be seen in IDDM patients. SC32 (0. 058) and SC22 (0.047) were found with more high frequency in normals, and also seen in some healthy relatives of IDDM patients, but could not be seen in IDDM patient proper. Moreover, seventeen kinds of rare complotyes observed in normals have not been defected in IDDM patients, either.