紫外线减毒尾蚴活疫苗预防小鼠日本血吸虫感染有效免疫期的实验研究

The Effective Period of Immunization with UV-attenuated Living Cercariae Vaccine against S.Japonicum Infection in Mice

以 400μW/(min·cm^2)剂量紫外线照射减毒日本血吸虫尾蚴, 1 次免疫小鼠(200条/只)可产生抗日本血吸虫感染的免疫能力。这种免疫力至少可维持7个月。1、3、5、7个月各组中,免疫组与攻击组虫荷均数比较,差异均有显著性意义。1、3、5和7个月所获减虫率分别为71.28％、69.63％、62.26％和46.16％,7个月与1、3、5个月比较,差异有显著性意义,提示7个月时小鼠抗日本血吸虫的免疫力可能有所下降。

Effective resistance against 5. japonicum was induced by prior exposure to ultraviolet-attenuated cercariae of S. japonicum with 400 μW/ (min · cm2) on mice in single time. The results showed that the resistance against 5. japonicum could last at least seven months, as in one-month group, three-month group, five-month group, and seven-month group the mean worm burden of immune groups was significantly less than that of challenge groups. The reduction percentage of worm burden (RPWB) was 71.28%, 69.63%, 62.26% and 46.1 % of immunization after one, three and five months respectively, and there is a significant difference between the one-month, three-month and five-month groups and the seven-month group, whose RPWB was 46. 16 %. So it seems reasonable to assume that the resistance against S. japonicum in the seven-month group has declined slightly.