常通口服液预防术后肠粘连的实验研究

Changtong oral liquid for prevention of postoperative intestinal adhesion: an experimental study

目的观察中药常通口服液用于术后腹腔肠粘连的效果。方法选取54只SD雄性大鼠随机分为6组(n=9):正常对照组、模型对照组、四磨汤组、常通口服液低、中、高剂量组。除正常对照组外,其余大鼠均按Ellis法制备成肠粘连模型。正常及模型对照组予以蒸馏水灌胃(10ml/kg);四磨汤组以10ml/kg灌胃给药;常通口服液低、中、高剂量组分别按4.3、8.6、17.2g/kg灌胃给药。各组于术后第7天取血,测定白细胞(WBC)计数及纤维蛋白原(FIB)浓度;同时进行粘连程度肉眼分级。结果常通口服液能明显减轻肠粘连程度,降低大鼠血浆中WBC计数及FIB浓度。结论常通口服液能够预防腹腔术后肠粘连形成。

Objective To observe the effect Changtong oral liquid (CTOL), a traditional Chinese drug preparation, on the prevention of postoperative intestinal adhesion. Methods Fifty-four male SD rats were randomly divided into 6 equal groups, namely the normal control, model control, Simo decoction (SMD, another traditional Chinese drug preparation), and CTOL groups, and CTOL group included three subgroups treated at low, moderate and high doses. Except for those in the normal control group, all the other rats were subjected to operation with Ellis' method for establishing intestinal adhesion models. After the operation, the rats in the normal control and model groups received intragastric administration of distilled water (10 ml/kg), SMD group received SMD administration in similar manner at the dose of 10 ml/kg, and the three CTOL groups had CTOL at the doses of 4.3, 8.6 and 17.2 g/kg, respectively. On day 7 after surgery, blood sample were taken from all the rats to determine the white blood cell (WBC) count and fibrinogen (FIB) concentrations, and the intestinal adhesion was graded. Results and Conclusion CTOL evidently reduced the severity of postoperative adhesion and decreased WBC count and plasma FIB level, suggesting the efficacy of CTOL in inhibiting the formation of postoperative intestinal adhesion.