FT3^M,FT4^M磁性固抗放射免疫分析方法学研究

RADIOIMMUNOASSAYS FOR FREE TRIIODOTHYRONINE AND FREE THYROXINE IN SERUM WITH MAGNETIC SEPARATION OF THE SOLID PHASE

FT_3~M、FT_4~M磁性固抗放射免疫分析法是采用特定的磁性固相抗体,集提取和分离为一体的一种灵敏而快速的直接二步法(反滴定法)。液体血清标准的适宜温育条件,用于直接测定人血清游离甲状腺激素(FT_3、FT_4)浓度。

Two sensitive and rapid RIA method for quantitative measurement of free triiodothyronine (FT_3) and free thyroxine (FT_4) in serum are described. These two step RIA procedures utilize immunoextraction of free hormone and separation of free phase by specific, high affinity polyclonal antiserum coupled to cellulose coated magnetic iron oxide particles. The measurement range is 0—100 pmol/l for FT_4 and 0—50 pmol/l for FT_3 respectively. Sensitivity of FT_3 and FT_4 is no more than 0.5 pmol/l. The normal range is 2.5—9.5 pmol/l for FT_3 and 9.5—25.5 pmol/l for FT_4.