环氧化酶-2及COX-2m RNA在脑膜瘤中的表达及意义

Expression of COX-2 and COX-2mRNA in Meningioma and Its Significance

目的观察环氧化酶-2(COX-2)蛋白及COX-2mRNA在人脑膜瘤组织中的表达,探讨COX-2基因表达与脑膜瘤病理分型的相关性.方法收集1995年3月～2003年4月在我院住院的56例脑膜瘤病人经手术治疗切除的肿瘤标本,另选颅脑损伤内减压手术切除的脑组织标本8例作为正常对照标本.石蜡包埋切片后,用免疫组化的方法检测COX-2蛋白在脑膜瘤中的表达,用原位杂交的方法检测COX-2mRNA在脑膜瘤中的表达.结果脑膜瘤的COX-2蛋白和COX-2mRNA的阳性表达率分别为53.57%和58.93%;正常对照组不表达.恶性脑膜瘤的COX-2蛋白和COX-2mRNA表达率明显高于良性脑膜瘤(P＜0.01).结论COX-2和COX-2mRNA在脑膜瘤中高度表达,且与脑膜瘤的病理分型有关;从蛋白水平和基因分子水平阐明COX-2可能在脑膜瘤的发生、发展过程中起重要作用.

Objective To observe the expression of Cyclooxygenase-2 (COX-2) and COX-2mRNA in human meningioma and to explore the relation between the COX-2 expression and the pathology classification of meningioma. Methods The expression of COX-2 was detected in 64 cases of meningioma and brain tissue by immunohistochemistry method, and the expression of COX-2mRNA was examined by in situ hybridization. Of the 64 cases, 21 cases were from human malignant meningioma, 35 cases were from the benign meningioma and 8 cases were normal controls.Results The expressions of COX-2 and COX-2mRNA in meningioma were (53.57%) and (58.93%), respectively; there is no expression in normal controls. The expressions of COX-2 and COX-2mRNA in malignant meningioma were significantly higher than in benign meningioma (P<(0.01)).Conclusion The COX-2 and COX-2mRNA expression was high in meningioma and significantly correlated with the pathologic classification of meningioma. COX-2 gene may play a important role in prompting the angiogenesis which contribute to the tumor genesis and progression in meningioma.