多聚乙烯亚胺介导小鼠IL-12基因转染人骨肉瘤细胞的细胞

Polythylenimine AS A Nonviral Vector for Murine Interleukin-12 Gene Transfer to the Mouse Osteosarcoma Cells

目的 确定多聚乙烯亚胺(polyethylenimine,PEI)最佳的转染条件。以 PEI为载体,将 mIL-12基因导入MG63细胞。方法 以MTT法检测PEI的细胞毒性。以PEI为载体,在不同的条件下,将荧光素酶基因和mIL-12基因导入 MG63 细胞,分别检测其转染效率。结果 PEI的细胞毒性较低。当N/P比值为8时,PEI的转染效率最高。PEI可以成功的将 mIL-12 基因导入 MG63 细胞。结论 PEI是一种低毒性、高效率的DNA载体,可以广泛应用于体内外基因治疗的试验。

Objective To investigate the best condition of polyethylenimine (PEI) for murine interleukin-12 gene transfer to MG63 cells.Methods The cytotoxicity of PEI was evaluated by MTT. In different conditions, using PEI as the vector,MG63 cells were transfected with luciferase gene and murine interleukin-12 (mIL-12) gene. Expression efficiency of the transduced gene was monitored.Results The cytotoxicity of PEI was low. When N/P ratio equalled to 8, the transfection efficiency was highest, and mIL-12 gene could also be successfully transferred to MG63 cells with PEI as the vector.Conclusion PEI is low cytotoxicity, and high efficiency vector. It can be widely used in the area of gene therapy.