利用示踪技术对高锌鸡蛋疗效机理的研究及临床意义

CLINICAL SIGNIFICANCE AND MECHANISM OBSERVATION OF THERAPEUTIC EFFICIENCY OF HIGH ZINC EGGS BY MEANS OF TRACER TECHNIQUES

为进一步探讨高锌鸡蛋与硫酸锌对小儿低锌血症不同疗效和治疗机理,我们以小鼠的肝、肾作为研究对象,分实验组和对照组采用三种较先进的技术手段:①以^(65)Zn为示踪剂,通过闪烁测量,分析S^(65)Zn在肝、肾中的吸收分布;②用光显微自显影技术,观察^(65)Zn在肝、肾中的定位;③用钙钴法显示碱性磷酸酶活性呈色反应强度,了解酶的活性强弱。通过上述系列相关的研究,可在两组中比较两种锌在临床应用上的不同意义,为阐明食用高锌鸡蛋对小儿低锌血症特殊疗效价值,提供基础理论依据。

The report describes the results of laboratory observations of liver and kidney in mice body by the following methods: (1) scintillation measurement for liver and kidney samples of mice, which feed on yolk of eggs labelled with ^(65)Zn, (2) light microscopic-autoradiograph (ARG) observation of liver and kidney samples labelled with ^(65)Zn and (3) immuno-histochemistry staining analysis by calcium-cobalt method for AKP enzymes of liver and kidney samples. Results indicated that ^(65)Zn have been integrated with yolk of egg in the course of biological-conversion, making up a metal protein (metallothioneine) and offering advantageous conditions for absorption utilization of zinc in the body. The study provides theoretical basis for clinical applications of high-zinc eggs.