脊髓损伤后血清和脑脊液中S-100B蛋白的动态变化

The dynamical assays of S-100B proteins in serum and cerebrospinal fluid after acute spinal cord injuries

目的 研究急性脊髓损伤(spinalcordinjury, SCI)后血清和脑脊液中S 100B蛋白的动态变化及其意义。方法 用Allen打击法制作狗的急性脊髓损伤模型,于损伤后不同时间采集血液和脑脊液,用酶联免疫吸附法 (ELISA)检测其中的S 100B含量,与对照组比较研究其变化规律。结果 急性脊髓损伤后血清和脑脊液中S 100B含量均显著升高且其水平呈动态变化,S 100B含量于伤后 2h即显著升高, 6h达高峰, 10h后降至正常,而且血清和脑脊液中两者含量平行。结论 血清和脑脊液中S 100B蛋白于脊髓损伤后显著增高,有望成为急性脊髓损伤检测的标志性蛋白之一。

Objective To study the dynamical changes of S-100B proteins in serum and cerebrospinal fluid (CSF) after acute spinal cord injuries (ASCI) and the clinical significance. Methods Dog models with ASCI were made by the method of Allen's attack. The levels of S-100B in serum and CSF were measured by enzyme-linked immunosorbent assay (ELISA) at different times after ASCI, and the principle of changes was studied and compared to that of the control group. Results The contents of S-100B in serum and CSF increased significantly at 2h after ASCI, mounted up to the summit at 6h and dropped to the normal at 10h. The content in serum was parallel to that in CSF all the time. Conclusion The levels of S-100B proteins in CSF and serum increase significantly after SCI. S-100B proteins can be seen as the marker for ASCI and further may be studied as the measurement to assess the severity and prognosis of ASCI.