一氧化氮在梗阻性黄疸大鼠肾功能损害中的作用

目的 探讨一氧化氮 (NO)在梗阻性黄疸大鼠肾功能损害中的作用。 方法 雄性SD大鼠 3 6只随机分为对照组 (A组 )、胆总管结扎 7d组 (B组 )和第 2 1天组 (C组 ) ,每组 12只。分别检测各组血清总胆红素、胆红素、ALT、肌酐、尿素氮和血浆NO。光镜观察肾脏组织的病理改变。 结果 B组胆红素、ALT和NO较A组显著升高 (P <0 .0 1) ;C组胆红素、ALT、肌酐和尿素氮较A组显著升高 (P <0 .0 1) ,NO却无明显变化。B组和C组部分肾小管上皮细胞肿胀和坏死 ,C组较严重。 结论 血浆NO水平与梗阻性黄疸大鼠肾功能损害程度不一致 。

Objective To explore the effect of nitric oxide(NO) on renal function of rats with obstructive jaundice. Methods 36 SD male rats were randomly divided into 3 group: control group(A), 7th day of common biliary duct ligation(CBDL) group(B) and 21th day of CBDLgroup(C). The serum total bilirubin, bilirubin, ALT, creatinine, blood uria nitrogen and NO were detected respectively and the pathological changes of kidney under light microscope were observed as well. Results The levels of serum bilirubin, ALT and NO of group B and serum bilirubin, ALT, creatinine and blood uria nitrogen of group C were significantly higher than those of group A(P<0.01). But the serum NO levels of group C were not significant difference compared to those of group A. The renal tubule endothelium appeared swelling and necrosis in part rats of group B and C, and the change was more serious in rats of group C. Conclusion The leves of serum NO were not correlation with the degree of rats renal function injury. NO may play different effect on renal function in different periods of obstructive jaundice.