摘要
该文报道了硫堇(Tn)在玻碳电极上的电化学聚合,用原子力显微镜,紫外可见光谱,傅立叶红外变换光谱等手段对纳米聚硫堇进行了表征;研究了纳米聚硫堇修饰电极的电化学性质及血红蛋白在该修饰电极上的直接电化学行为。实验结果表明,纳米聚硫堇修饰电极对血红蛋白(Hemoglobin,Hb)的还原具有催化作用,且还原峰电流与被测蛋白质浓度呈良好线性关系。流动注射分析结果进一步表明该纳米修饰电极具有高的稳定性和好的重现性,用该法测定人体血液中血红蛋白,获得了满意的结果。
A poly(thionine) nanoparticles modified glassy carbon electrode had been prepared by a novel two-step eleetrepolymerization method. Atomic force microscopy (AFM), UV-vis spectrum and Fourier Transform Infrared Spectrometry (FTIR) were used to evaluate poly(thionine) nanoparticles. The electrochemical properties of poly (thionine) nanoparticles as well as the electrochemical behaviors of hemoglobin(Hb) on PTn/GCE had been studied. The results showed that the modified electrode exhibited significantly electroeatalytie activity for the reduction of Hb, and the reduction currents were linear to the concentrations of Hb in aqueous solutions. In addition, the results of flow injection analysis (FIA) further suggested high stability and reproducibility of PTn/GCE and this method was applied to the determination of hemoglobin (Hb) of whole blood.
出处
《化学传感器》
CAS
2004年第4期17-24,共8页
Chemical Sensors
基金
国家自然科学基金(No.20175006)资助项目
