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基于电聚合膜/溶胶-凝胶固定酶的葡萄糖传感器的研究 被引量：1

Glucose biosensor based on electropolymerization/sol-gel matrix
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摘要 该文以铂电极为基底,电聚合乙酰苯胺作为修饰膜,采用氧化锆溶胶-凝胶法固定酶制备葡萄糖酶传感器。探讨了酶的浓度,溶液的pH值等对电极响应的影响,考察了电极的重现性,干扰及使用寿命。该方法有效的排除了尿酸(UA),抗坏血酸(AA)对测定的干扰。该传感器在葡萄糖浓度8.2×10^(-6)～8.0×10^(-3)mol/L范围内有线性响应,检出限为4.1×10^(-6)mol/L。对葡萄糖有较好的响应特性、稳定性和使用寿命。 A glucose biosensor has been prepared based on poly (N-acetylaniline) (PNAANI)modified pt electrode and the glucose oxidase is immobilized in ZrO_2 sol-gel matrix. The effects of experimental conditions such as glucose oxidase concentration, pH values on the electrode performance have been investigated, and the reproducibility, interferences and lifetime of the electrode are also tested. The biosenser effectively excludes the interferences of UA and AA. The optimal electrode gives a linear response range of 8. 2×10^(-6)～8.0×10^(-3) mol/L for glucose concentration and detection limit (S/N=3) of 4. 1×10^(-)6 mol/L.
作者 渠凤丽 阳明辉 杨云慧 沈国励 俞汝勤
机构地区 湖南大学化学化工学院化学生物传感与计量学国家重点实验室
出处 《化学传感器》 CAS 2004年第4期41-46,共6页 Chemical Sensors
基金 国家自然科学基金(No.20075006) 博士点基金(No.20010532008)
关键词 固定 UA 葡萄糖浓度 尿酸 AA 抗坏血酸 影响 电聚合 溶胶-凝胶 铂电极 N-acetylaniline glucose biosensor electropolymerization
分类号 TP212.3 [自动化与计算机技术—检测技术与自动化装置]
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参考文献15

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共引文献8

同被引文献24

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二级引证文献10

化学传感器
2004年 第4期

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