摘要
该文以中性红为核,二氧化硅为壳,利用反相微乳液技术,通过正硅酸四乙酯的水解制备了掺杂有中性红的二氧化硅(NRDS)纳米颗粒。将NRDS纳米颗粒与L-谷氨酸氧化酶(L-GLOD)混合,再通过戊二醛交联,得到以中性红为媒介体的L-谷氨酸生物传感器。该传感器不仅克服了传统介体型传感器中介体易流失的缺点,更提高了检测的灵敏度。实验证明,该传感器对L-谷氨酸的线性检测范围为1.0×10^(-7)～1.5×10^(-4)mol/L,检出限为5.0×10^(-8)mol/L。与微渗析技术联用,成功地用于正常大鼠和患糖尿病大鼠脑中L-谷氨酸的检测,为生理学以及临床医学的研究提供了新的检测手段。
In this paper, microdialysis sampling was combined with enzymatic assay of L-glutamate level in the brain of Ⅰ type diabetic rat. A novel amperometric L-glutamate biosensor based on Neutral red-doped silica (NRDS) nanoparticles was developed. These uniform NRDS nanoparticles (about 45nm±3nm) were prepared by a water-in-oil (W/O) microemulsion method, which were characterized by TEM and electrochemical technology. The nano silica surface exhibited high biocompability and the doped inside neutral red maintained its high electron- transfer efficiency as a mediator. The L-glutamate biosensor showed a detection limit of 5.0×10^(-8) mol/L with the linear range from 1.0×10^(-7) mol/L to 1.5×10^(-4) mol/L. Coupled to microdialysis, it was used to determine the L-glutamate concentration in the brain of normal and Ⅰ type diabetic rat. The result was in satisfactory agreement with the references.
出处
《化学传感器》
CAS
2004年第4期47-52,共6页
Chemical Sensors
基金
国家自然科学基金(No.20175006)
博士点基金(No.20030269014)
华东师范大学2004年优秀博士生培养基金资助课题
