胃癌同证型中肿瘤转移相关基因蛋白的表达

Expression of tumor metastasis-related gene protein in the same syndromic classification of gastric carcinoma on traditional Chinese medicine

目的:从基因蛋白表达层次探索胃癌中医证本质. 方法:从第二军医大学长征医院普外科收集术前胃癌患者, 并按中医辨证分型标准将其归属;取术后肿瘤标本,用免疫组化方法检测胃癌肿瘤中E-Cadherin、C-erbB-2、P53、nm23、ICAM-1、VEGF、KDR、MMP-2、TIMP- 29种基因蛋白表达情况. 结果:每个证型中的9个基因蛋白之间均有不同表达,统计学分析表明6证型的9个基因蛋白表达均有显著性差异P=0.0 001,进一步两两比较示同一证型内部多组基因都存在表达差异性;VEGF、E-cad、nm23表达率较高,分别为94%、90%、92%.痰湿凝结型中E-cad的蛋白表达最高,以平均秩和表示为:63.09,肝胃不和、瘀毒内阻两型E-cad表达较低. 结论:肝胃不和、瘀毒内阻两型胃癌发生转移可能主要与E-cad的缺失有关,但内部既有癌基因的高表达亦有抑癌基因高表达.

AIM: To explore the entity of gastric carcinoma under the same syndromic classification based on traditional Chinese medicine from the angle of gene protein expression. METHODS: Patients with gastric carcinoma were collected and then classified before operation according to dialectic criterion of traditional Chinese medicine. Samples of gastric carcinoma from all the patient were collected after operation. Envision two step immuhistochemical method was used to observe the expession of E-cadherin, C-erbB-2, P53, nm23, ICAM-1, VEGF, KDR, MMP-2, TIMP-2 in the cancerous samples. RESULTS: The preceding 9 gene proteins were differently expressed in each type . There was significant difference (P=0.0 001<0.01) in the expression of the 9 gene proteins between different syndromic types. Several genes had different expression in the same syndromic type. The rates of VEGF, E-cad, nm23 expression were higher (94%, 90% and 92% respectively) than other proteins. E-cad expression in the stagnation of phlegm-damp type is the highest expression (average rank = 63.09). E-cad expression was lower in the types of the stasis and endogenous toxics and the discordance of liver-stomach. CONCLUSION: Metastasis of gastric carcinoma in the types of the stasis and endogenous toxics and the discordance of liver-stomach may relate to the loss of E-cadherin expression. However, both cancer gene and anti-oncogene are expressed at a high level in the two types.