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应用表达谱芯片技术筛选HBcAg反式调节基因

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摘要 目的:筛选与克隆HBcAg激活基因,了解其在体内的调节功能线索及机制. 方法:以分子生物学技术构建HBcAg的真核表达载体pcDNA3.1(-)-HBcAg,以表达质粒pcDNA3.1(-)-HBcAg 转染HepG2细胞,以空载体pcDNA3.1(-)为平行对照,制备转染后的细胞裂解液,提取mRNA.应用基因表达谱芯片技术对差异表达mRNA进行检测和分析. 结果:HBcAg表达质粒pcDNA3.1(-)-HBcAg经酶切鉴定和DNA测序鉴定正确.经基因表达谱芯片分析,29种基因的表达水平上调,17种基因的表达水平下调. 结论:筛选到一些与细胞内信号传导、免疫调节、细胞凋亡、蛋白质翻译合成、肿瘤发生相关的HBcAg反式调节的靶基因.
作者 徐志强 成军 张鸿飞 王建军 刘妍 纪冬
机构地区 中国人民解放军第
出处 《世界华人消化杂志》 CAS 2004年第12期2886-2890,共5页 World Chinese Journal of Digestology
基金 国家自然科学基金攻关项目 No.C03011402 No.C30070689军队"九 五"科技攻关项目 No 98D063军队回国留学人员启动基金项目 No.98H038军队"十 五"科技攻关青年基金项目 No.01Q138军队"十 五"科技攻关面上项目 No.01MB135
关键词 表达谱芯片技术 筛选 HBCAG 反式调节基因 分子生物学技术
分类号 R346 [医药卫生—基础医学]
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参考文献32

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世界华人消化杂志
2004年 第12期

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