苯酚-硫酸法测定多糖含量显色方式的优选

Optimization of the Way of Coloration in the Process of Determining Polysaccharide Content by Phenol-Sulfuric Acid Method

目的 优选在以葡萄糖为对照时,苯酚-硫酸法测定多糖含量的显色方式。方法 采用正交试验法。结果 对正交试验结果进行直观分析和方差分析,优选显色方式为:取待测溶液 2.0 mL,加入 5%苯酚液 1.0 mL,混匀,迅速滴加浓硫酸 7.5 mL,摇匀,室温放置 15 min,然后置冷水中冷却 30 min,在 490 nm 波长处测定。结论 优选的显色方式操作更简便、易行。

Objective To optimize the way of coloration in the process of determining polysaccharide content by phenol-sulfuric acid method, using only glucose as the standard. Method By orthogonal test. Results After synthesizing the results of direct analysis and variance analysis, the optimum way of coloration was obtained as follows: 1.0 mL 5% phenol solution was added to 2.0 mL testing solution and mixed. Then 7.5 mL concentrated sulfuric acid was added quickly drop by drop. After set at ambience for 15 min and cooled following in cold water for 30 min, the solution was detected at wavelength of 490 nm. Conclusion The optimum way of coloration can be easily controlled and operated.