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三种检测扎幌样病毒方法的比较分析 被引量：1

Comparative analysis of three methods for detection of Sapporo-like virus.
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摘要 目的 比较三种常用检测扎幌样病毒的方法,以选取适合我国标本的较优检测方法。 方法 对收集到的169份粪便分别采用三种逆转录聚合酶链反应(RT-PCR)方法进行检测,扩增产物进行测序分析。 结果 方法A、B、C检测阳性标本数分别为3份(排出诺瓦克病毒阳性标本后)、4份、1份。4份PCR产物测序分析只有1份为扎幌样病毒,其余为轮状病毒。 结论 方法C特异性最好,即半套式RT-PCR优于其它二种RT-PCR方法。 Objective To compare three methods for detection of Sapporo-like virus and selection of better one. Methods Stool specimens were tested by three RT-PCR methods, respectively, and amplified products were sequenced and analyzed. Results The number of positive specimens was 3,4 and 1 by the three methods,respectively.Only one can be determined to be Sapporo-like virus in four sequenlially amplified products,and the other three were rotavirus. Conclusion The third method or the semi-nested RT-PCR is better for detection of Sapporo-like virus.
作者 刘翼 戴迎春 李建栋 詹惠春 聂军
机构地区 南方医科大学流行病学教研室
出处 《中国热带医学》 CAS 2005年第1期7-8,52,共3页 China Tropical Medicine
基金 广东省医学科研基金(A2003420)
关键词 扎幌样病毒 测序分析 阳性标本 逆转录聚合酶链反应 轮状病毒 RT-PCR方法 套式RT-PCR 扩增产物 粪便 排出 Sapporo-like virus/SLV Rotavirus Reverse transcriptase polymerase chain reation/RT-PCR
分类号 R511 [医药卫生—内科学] R373.2 [医药卫生—病原生物学]
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参考文献5

  • 1Jiang X, Huang PW, Zhong WM, et al. Design and evaluation of a primer pair that detects both Norwalk and Sapporo-like caliciviruses by RT-PCR[ J] . J Virol Methods, 1999,83:145 ～ 154.
  • 2Vinje J, Deijl H, vander Heide R, et al. Molecular detection and epidemiolo gy of Sapporo-like virus[J]. J Clin Microbiol,2000,38:530 ～ 536.
  • 3Hansman GS, Datayama K, Maneekarn N, et al. Genetic diversity of norovirus and sapovirus in hospitalized infants with sporadic cases of acute gastroenteritis in Chiang Mai, Thailand [ J]. J Clin Microbiol, 2004, 42 (3): 1305 ～ 1307.
  • 4刘翼,戴迎春,李建栋,詹惠春,陈清,俞守义,聂军.广州地区扎幌样病毒的检出及基因型分析[J].第一军医大学学报,2004,24(10):1147-1149. 被引量：8
  • 5Schuffenecker I, Ando T, Thouvenot D, et al. Genetic classification of "Sapporo-like viruses" [J]. Arch Virol, 2001,146: 2115 ～ 2132.

二级参考文献8

  • 1Green KY, Ando T, Balayan MS, et al. Taxonomy of the caliciviruses[J]. J Infect Dis, 2000, 181(s2): s322-30.
  • 2Chiba S, Nakata S, Numata-Kinoshita K, et al. Sapporo virus: history and recent findings[J]. J Infect Dis, 2000, 181 (s2): s303-8.
  • 3Hansman GS, Katayama K, Maneekarn N, et al. Genetic diversity of norovirus and saprovirus in hospitalized infants with sporadic cases of acute gastroenteritis in Chiang Mai, Thailand [J]. J Clin Microbiol, 2004, 42(3): 1305-7.
  • 4Schuffenecker I, Ando T, Thouvenot D, et al. Genetic classification of "Sapporo-like viruses" [ J ]. Arch Virol, 2001, 146( 11 ): 2115-32.
  • 5Pang XL, Honma S, Nakata S, et al. Human caliciviruses in acute gastroenteritis of young children in the community[J ]. J Infect Dis,2000, 181(s2): S288-94.
  • 6Phan TG, Okame M, Nguyen TA, et al. Human astrovirus, norovirus(GZ, GII), and sapovirus infections in Pakistani children with diarrhea[ J ]. J Med Virol, 2004, 73(2): 256-61.
  • 7Rockx B, de Wit M, Vennema H, et al. Natural history of human calicivirus infection: a prospective cohort study [ J ]. Clin Infect Dis,2002, 35(3):246-53.
  • 8陈冬梅,张又,钱渊,杨松.北京地区婴幼儿人类杯状病毒感染状况及型别分析[J].中华儿科杂志,2002,40(7):398-401. 被引量：60

共引文献7

同被引文献7

  • 1HANSMAN G S, OKA T, KATAYAMA K, et al. Human sapoviruses: genetic diversity, recombination, and classification[J]. Rev Mecl Virol, 2007, 17(2): 133-141.
  • 2WU F T, JIANG D D S, MUO C H, et al. First sapovirus outbreak in Taiwan[J]. Epidemiol Bull, 2007, 23(11): 325-334.
  • 3OKA, T, KATAYAMA K, HANSMAN G S, et al. Detection of human sapovirus by real-time reverse transcription-polymerase chain reaction [J]. J Med Virol, 2006, 78(10): 1347-1353.
  • 4HANSMAN G S, TAKEDA N, KATAYAMA K, et al. Genetic diversity of sapovirus in children, Australia[J]. Emerg Infect Dis, 2006, 12(1): 141-143.
  • 5HANSMAN G S, KATAYAMA K, OKA T, et al. Mutational study of sapovirus expression in insect cells[J]. Virol J, 2005, 2: 13.
  • 6KINGSLEY D H, MEADE G K, RICHARDS G P. Detection of hepatitis A virus and Norwalk-like virus in imported clams associated with food-brone illness[J]. Applied and Environmental Microbiology, 2002, 68(8): 3914-3918.
  • 7萨姆布鲁克 J,弗里奇 E F,曼尼阿蒂斯T.分子克隆实验指南[M].2版.金冬雁,黎孟枫,译.北京:科学出版社,1999:237-262.

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中国热带医学
2005年 第1期

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