连接酶链反应检测结膜沙眼衣原体

Detection of Clamydia trachomatis with ligase chain reaction.

目的了解活动性沙眼患者的年龄分布,评价WHO简易沙眼分级法和连接酶链反应(LCR)诊断沙眼衣原体的意义.方法根据WHO简易沙眼分级法,对89例受检者进行沙眼分级,并应用刮擦拭子采集上睑结膜标本,进行连接酶链反应以诊断沙眼衣原体.结果10岁以下的沙眼占56.18%,20岁以下占26.94%;84例沙眼及可疑沙眼中,16例LCR阳性(19.05%),各抽样点的LCR阳性率为7.14%～34.38%. 结论应加强对青少年活动性沙眼的防治;WHO的简易分级活法适合在基层农村推广;连接酶链反应可用于早期沙眼的诊断及批量样本的检测,需注意采样方法及转送温度条件的控制.

Objective To investigate the age distribution of the active trachoma and evaluate the significance of trachoma classi-ficltion and ligase chain reaction. Methods 89 cases were investigated and classified by WHO trachoma classification, samples of the superior conjunctiva were collected for detection of Chlamydia trachomatis with LCR. Results Trachoma is more common in the age group below 10-year-old (56.18% ) .It is 26.94% in the age group below 20-year-old.Out of 84 trachoma and suspected trachoma,16 cases are LCR positive(19.05% ) .The positive rates in various sites are 7.14% - 34.38% . Conclution Attention be paid to the prevention and treatment of adolescent trachoma; WHO trachoma classification is easy and feasible in the countryside . Ldgase chain reaction could be used for early diagnosis and the detection of a large number of sampes. Attention be paid to the collection of samples and the temperature of transportation should be controlled carefully.