烫伤大鼠复苏前后小肠组织凋亡基因表达的变化

Changes in the expression of apoptotic genes in the intestinal tissue of scalded rats before and after resuscitation

目的观察烫伤大鼠复苏前后小肠组织中原癌基因cfos、增殖细胞核抗原(PCNA)及凋亡促进基因Bax蛋白等表达的变化规律。方法采用Wistar大鼠30%TBSAⅢ度烫伤模型,根据伤后观察时间分为复苏前(伤后2.0h,未补液)、复苏后0.5h(伤后2.5h)、2.0h(伤后4.0h)、4.0h(伤后6.0h)组,每组8只。伤后2.0h开始,后3组大鼠常规补液抗休克。4组大鼠于不同时相点放血致死,留取小肠组织标本用以检测cfos、PCNA及Bax的表达。结果cfos、PCNA及Bax在烫伤大鼠复苏前的表达水平分别为(65.8±4.2)%、(74.5±2.4)%、(26.3±5.7)%;复苏后0.5、2.0h3种基因表达均有明显增加,其中0.5h达高峰,分别为(92.4±5.7)%、(85.6±4.5)%、(67.1±66)%,与复苏前比较,差异有统计学意义(P<0.01);复苏后4.0h各基因表达与复苏前比较,差异无统计学意义(P>0.05)。结论严重烫伤复苏早期,大鼠小肠组织凋亡基因表达显著增加。

Objective To explore the dynamic changes in the expression of c-fos, proliferation cell nuclear antigen (PCNA) and Bax in the intestinal tissue of scalded rats before and after resuscitation. Methods Wistar rats inflicted with 30% TBSA full thickness scald were employed as the model and randomly divided into four groups with 8 in each group, i.e. 2.0, 2.5, 4.0, 6.0 postscald hour (PSH) groups. Rats in each group received routine fluid infusion at 2 PSH, and were sacrificed at 2, 2.5, 4, 6 PSH, respectively. Then the intestinal tissue of the rats was harvested for the detection of the expression of c-fos, PCNA and Bax. Results The expression of c-fos, PCNA and Bax at 2.0 PSH group (65.8±4.2%, 74.5±2.4%, 26.3±5.7%,respectively) significantly increased when compared with those in 2.5 PSH group (92.4±5.7%, 85.6±4.5%, 67.1±6.6%, respectively) (P<0.01). The expression of 3 genes increased dramatically at 2.5 and 4.0 PSH, and reached the peak at 2.5 PSH. There was no obvious difference in the gene expression between 4 PSH and 2 PSH groups. Conclusion The expression of apoptotic genes in the intestinal tissue of scalded rats increased significantly during early resuscitation stage after burn injury.