广东地区SARS-COV细胞培养模型的建立和初步应用

Establishment and Application of Cell Model Infected by SARS Coronavirus in Guangdong

目的 :建立中国广东地区SARS-CoV细胞培养模型。方法 :通过将SARS -CoV广东地区分离株GD32 2在VeroE6细胞中连续传代 ,检测TCID5 0 ,获得稳定的细胞模型。利用此细胞培养模型 ,对不同温度、紫外线等条件下SARS -CoV的存活时间进行了比较。对重组人复合干扰素α抗病毒效果进行了筛查。结果与结论 :SARS -CoV对外界环境抵抗力比普通冠状病毒强 ,对重组人复合干扰素α有较高的敏感性 ,抑制指数达 4 .9。

Objective:To establish a cell model of SARS coronavirus (SARS-CoV) in Guangdong.Methods:The Vero E6 cells were consistently infected by SARS-CoV GD322 and TCID_(50) were assayed.The surviving time of SARS-CoV GD322 were compared in different temperatures and ultraviolet ray.The antiviral effect of rIFN-α was studied.Results and Conclusion:The resistance of SARS-CoV to conditions was stronger than the other coronavirus.The rIFN-α was effective in SARS-CoV and the inhibit index was 4.9.