产1,3-丙二醇菌株丁酸梭菌的诱变育种

Breeding of Strain Clostridium butyricum Producing 1,3-Propanediol

甘油由丁酸梭菌转化成 1,3-丙二醇的研究是在厌氧条件下进行。为了获得 1,3-丙二醇的高产突变株 ,以丁酸梭菌为出发菌株进行诱变处理。经过硫酸二乙酯 (DES)化学诱变得到 2株高产正突变株C .but2 0 31和C .but2 0 4 6 ,再经过紫外线和亚硝基胍 (NTG)复合诱变得到突变株C .but30 37。经过初筛、复筛和传代实验 ,表明其是稳定的突变株。C .but 30 37的 1,3-丙二醇产量由出发菌株的 2 .2g L提高到 15 .7g L ,提高了 6 .13倍。

The conversion of glycerol to 1,3-propanediol (1,3-PD) by Clostridium butyricum was studied in anaerobic culture.Clostridium butyricum,as original strain,was treated twice by diethyl sulfate and two mutants,C.but 2031 and C.but 2046,were obtained.Then they were treated by multiple mutagenesis UV and N-methyl-N'-nitro-N-nitrosogunidine(NTG).A higher-yield strain C.but 3037 was bred through the primary screening and examination test of 1,3-PD production as the secondary screening.It was showed that the genetic property of this new mutant was stable by pass-generation tests and the yield of 1,3-PD was higher than 15.7g/L,which had been increased about 631% than that of the initial strain.