高效液相色谱法测定齐多夫定胶囊中AZT的含量

Determination of AZT in AZT Capsule by HPLC

目的 建立齐多夫定胶囊中 AZT的含量测定方法。方法 高效液相色谱法 ,Nova-Pak○RC1 8色谱柱 ( 60 A0 4μm,3 .9× 15 0 mm) ;流动相 :甲醇 -水 ( 2 0∶ 80 ) ;流速 :1.0 m L· min- 1 ;检测波长 :2 65 nm。 结果 齐多夫定进样量在 0 .2～ 2 .0 μg范围内呈良好的线性关系 ( r=1.0 0 0 0 ,n=5 ) ,重复性良好 ,RSD=0 .96% ( n=6) ,平均回收率 10 0 .1% ,RSD=1.49% ( n=9)。 结论 本法快速、简便、准确。

OBJECTIVE To establish a method for determination AZT in AZT Capsule.METHODS The analysis column was Nova-Pak ○RC 18(3.9×150mm 4μm);and the mobile phase was CH 3OH-H 2(20∶80)at a flow rate 1.0mL·min -1 the determination wavelength was 265nm。RESULTS In the scope of 0.2～2.0μg the contents of AZT had good linear relationship with their peak area(r=1.0000,n=5)。 The average recoveriy was 100.1%,RSD=1.49%(n=9).CONCLUSION The results showed that the method was rapid ,simpie excellent precision.