体内淋巴细胞微核形成与大肠癌恶性度的关系

Study of in vivo micronucleus formation in lymphocytes from the patients and its relation to malignant degrees of colorectal cancer

目的 探讨淋巴细胞微核形成与大肠癌恶性度的关系。方法 112例大肠癌患者术前取外周血进行体内淋巴细胞微核检测 ,实验数据用SPSS统计软件处理 ,比较各类型大肠癌自发微核的组间和良性大肠病变、健康人群对照组的差异。结果 各类型大肠癌组微核率 (micronucleusfrequency ,MNF)与对照组相比 ,MNF差异有显著性 (P <0 .0 1) ;随着患癌组分化度降低及淋巴结转移率的增加 ,MNF逐渐上升 ,并且低分化组与其它分化组之间 ,MNF差异有显著性 (P <0 .0 1)。结论 患者淋巴细胞微核形成与大肠癌恶性度密切相关 ,为大肠癌患者的术前恶性度的判断和高危人群筛查提供了一个有用的生物学标志物。

Objective To investigate the association of the micronucleus (MN) formation in lymphocytes from patients with the malignant degrees of colorectal cancer. Methods The MN test in capillary blood lymphocytes was conducted in 112 patients randomly selected from in-hospital patients before therapy. Experimental data were analyzed by SPSS (v.10.1) software. Results The differences in the frequency of MN between 7 pathological types of colorectal cancers and controls were statistically significant (P<0.01). The frequency of MN increased with the decrease of the histological differentiation in colorectal cancer, and the statistically significant differences were seen between low differentiation group and the other differentiation groups in colorectal cancers. Conclusion There is a significant correlation between MN formation and the malignant degrees of colorectal cancer, and MN formation will be a useful biomarker for the identification of malignant degrees of colorectal cancer before operation or for the screening of high risk subgroup.