食管鳞癌中COX-2 mRNA的表达以及NSAID对其的影响

COX-2 mRNA expression in esophageal squamous cell carcinoma before and after treated with non-steroidal anti-inflammatory drugs

目的:检测人类食管鳞癌组织和细胞中COX-2 mRNA的表达及NSAID对其的影响,探讨COX-2与食管鳞癌发病机制之间可能存在的关系. 方法:用逆转录聚合酶链反应法(RT-PCR)检测22例食管鳞癌患者液氮冻存的食管鳞癌组织和癌周正常食管鳞状上皮组织标本:COX-2 mRNA的表达.将人类食管鳞癌细胞株与阿司匹林或尼美舒利共同孵育后,用噻唑蓝法定量检测细胞增生情况,用RT-PCR法检测其COX-2 mRNA的表达情况. 结果:在22例食管鳞癌组织标本中有12例(54.5%)COX-2 mRNA表达阳性,但在癌周正常食管鳞状上皮组织标本均未检测到COX-2 mRNA表达.细胞培养结果表明,阿司匹林和尼美舒利对EC-9706细胞株的增生和COX-2 mRNA 表达均有影响;但对EC-109细胞株,仅阿司匹林对细胞的增生和COX-2 mRNA表达有影响. 结论:人类食管鳞癌常表达COX-2 mRNA,提示COX-2 有可能在食管鳞癌的发生机制中起重要作用,而且NSAID 很可能有助于预防和治疗此病.

AIM: To investigate the potential relationship between COX-2 mRNA expression and human esophageal squamous cell carcinoma (ESCC) and to explore the effect of non-steroi-dal anti-inflammatory drugs (NSAID) on ESCC. METHODS: Frozen specimens of human esophageal squamous cell carcinoma (n=22) and adjacent normal esophageal squamous epithelium were examined for COX-2 mRNA expression by reverse-transcription polymerase chain reaction (RT-PCR). After incubated with aspirin or nimesulide, proliferation of human esophageal squamous cancer cells (EC-9706 and EC-109) was quantified by MTT assay, and COX-2 mRNA expression in these cells was detected by RT-PCR. RESULTS: Of 22 cancer specimens, COX-2 mRNA was expressed in 12 cases(54.5%). However, in all the samples of adjacent normal esophageal squamous epithelium, COX-2 mRNA expression were not detected. Both aspirin and nimesulide inhibited EC-9706 cell proliferation and COX-2 mRNA expression. However, aspirin also inhibited EC-109 cell proliferation and COX-2 mRNA expression while nimesulide did not. CONCLUSION: COX-2 mRNA is frequently expressed in human ESCC and COX-2 may play an important role in carcinogenesis of ESCC. NSAID may be helpful in prevention and treatment of this cancer.