氧化苦参碱的抗肝纤维化作用及其机制研究

The Effects of Oxymatrine on Liver Fibrosis and its Mechanism

目的 观察氧化苦参碱的抗肝纤维化作用并探讨其作用机制。方法 采用四氯化碳诱导的小鼠肝纤维化模型观察氧化苦参碱干预前后肝指数、血、肝脏生化及病理组织学改变。比较治疗前后肝纤维化血清学检查指标变化 :透明质酸 (HA)、Ⅲ型前胶原肽 (PⅢP)、层连蛋白 (LN)、Ⅳ型胶原 (Ⅳ -C)。结果 在小鼠肝纤维化模型中 ,氧化苦参碱预防组较四氯化碳组的血清学指标 (HA、PⅢP、LN、Ⅳ -C)明显降低 (P <0 0 5 ) ,肝组织内SOD升高 ,MDA降低 ,病理组织学检查明显改善。结论 氧化苦参碱通过抑制肝内胶原合成、减少肝脏细胞外基质异常增生、降低自由基生成和减轻脂质过氧化发挥抗肝纤维化作用。

Objective To investigate the effects of oxymatrine on liver fibrosis and its mechanism. Methods The rat model of liver fibrosis induced by CCl 4 was set up. The effect of oxymatrine anti-liver fibrosis were evaluated by comparing liver weight index, serum/tissue biochemical indices and the change of tissue pathology before and after oxymatrine intervention. The levels of serum HA,PⅢP,LN and Ⅳ-C were also measured before and after oxymatrine treatment. Results In CCL4-induced liver fibrosis model rats, the levels of serum HA,PⅢP,LN and Ⅳ-C significantly decreased(P<0.05), liver SOD activity increased, MDA concentration was reduced, and liver pathological lesions obviously improved after oxymatrine treatment. Conclusion The data suggested that oxymatrine had prophylactic and therapeutic effects on the liver fibrosis probably through protecting hepatocyte, inhibiting fibrogenesis and fibrosis accumulation, and decreasing lipoperoxidation.