EPO对血透患者T淋巴细胞亚群及细胞因子的影响

The Effect of EPO on T Lymphocyte Subgroup and Cytokine in Hemodialysis Patients

目的 研究促红细胞生成素 (EPO)对维持性血透患者T淋巴细胞亚群及细胞因子的影响。方法 3 2例维持性血透患者作为观察组 ,2 0例正常人作为对照组。采用流式细胞仪检测正常对照组、维持性血透患者治疗前及EPO治疗后 3个月CD3 、CD4、CD8及CD4/CD8;采用ELISA检测其SIL -2R、IL -6、TNFα等细胞因子的变化。结果 EPO治疗 3个月后 ,CD4显著升高 (P <0 0 0 1) ,CD8、4/CD8降低 (P <0 0 5 ) ;SIL -2R、IL -6、TNFα等细胞因子均明显降低 (P <0 0 0 1)。结论 EPO可显著改善维持性血透患者的细胞免疫功能。

Objective To investigate the effect of EPO on T lymphocyte subgroup and cytokine in hemodialysis patients. Methods Thirty-two cases of patients underwent long-term hemodialysis were as experimental group, and 20 healthy subjects were as control group. The levels of CD 3, CD 4, CD 8 and CD 4/CD 8 were examined by flow cytometry in the both groups before and 3 months after EPO treatment, and the levels of SIL-2R, IL-6 and TNFα were detected by ELISA. Results CD 4 level significantly increased 3 months after EPO treatment (P<0.001), while the levels of CD 8,CD 4/CD 8, SIL-2R, IL-6 and TNFα significantly decreased(CD 4/CD 8:P<0.05; CD 8,SIL-2R,IL-6, and TNFα:P<0.001). Conclusion EPO could significantly improve T lymphocyte immunity function in patients underwent long-term hemodialysis.