纳米铁材料对小鼠血清生化指标的影响

Effect of Excess Iron Nano-particles on Serum Biochemical Indexes in Mice

[目的]探讨纳米铁材料对小鼠血清生化指标的影响。[方法]将CD小鼠随机分为对照组、纯铁粉组和纳米铁粉组。按照固定剂量(5g／kg)法对纯铁粉组和纳米铁粉组以纯铁粉或纳米铁粉一次经口灌胃。14天后脱髓处死。分别测定3组肝、肾脏器系数和血清生化指标的变化。[结果]实验小鼠全部存活。对照组、纯铁粉和纳米铁粉组的肝脏脏器系数分别为(0.0493±0.0062)、(0.0577±0.0048)和(0.0549±0.0041),纯铁粉和纳米铁粉组的肝脏脏器系数明显高于对照组(P<0.05)。对照组、纯铁粉组和纳米铁粉组总铁结合力(UIBC)和未饱和铁结合力(TIBC)分别为(12.65±9.82)和(77.50±7.19)μmol／L、(35.03±34.79)和(85.38±15.33)μmol／L及(8.93±7.39)和(71.67±11.87)μmol／L,与对照组相比纳米铁粉组血清UIBC和TIBC水平无明显差异(P>0.05),而纯铁粉组血清UIBC和TIBC与对照组相比有明显升高(P<0.05);纳米铁粉组血清葡萄糖(GLU)水平比对照组和纯铁粉组有明显降低(P<0.05),纳米铁粉组血清TBIL比对照组有明显降低(P<0.05),而纯铁粉组TBIL与对照组相比则无显著差异(P>0.05)。纯铁粉和纳米铁粉组血清乳酸脱氢酶(LDH)、总胆汁酸(TBA)均比对照组有明显降低(P<0.05);纳米铁粉组血清BUN明显高于纯铁粉组而肌酐(Cr)

[Objective] To explore the acute toxicity of iron nano-particles to mice.[Methods] The mice were treated with a single oral dose of iron nano-particles and iron powder at 5 g/kg body weight.After14 days,the mice were sacrificed and the blood were collected,and then the serum was used for biochemical analysis.[Results] Less abnormal behaviors were observed at 5 g/kg oral dose of iron nano-particles and iron powder in mice. The live/body coefficient in iron nano-particles and iron powder groups was obviously higher than that in control group(P<0.05).The serum unsaturated iron-binding capacity(UIBC)and total iron-binding capacity(TIBC) of iron nano-particles group were significantly lower than those of iron powder group(P<0.05),but compared with control group without statistical significance. The levels of serum TOIL and Glu of iron nano-particles group were significantly lower than those in control group (P<0.05).The levels of serum TBIL and Clu of iron powder have not any difference with control group.The serum LDH and TBA of bom iron nano-particles and iron powder groups were lower than those of control group. The serum BUN of iron nano-particles group was higher and serum Cr was lower compared with iron powder group. [Conclusion] In general, the iron nano-particles can be considered as less toxic and maybe has some special mechanisms of metabolism and toxicity compared with the iron powder of ordinary size.