高铁盐配位-分光光度法测定烟酰胺的含量

Spectrophotometry of Nicotinamide by coordination reaction of Ferric chloride

提出了测定烟酰胺含量的新方法 ,它是基于在碱性介质中烟酰胺与羟胺反应生成异羟肟酸 ,反应产物与 Fe3+反应生成紫红色配合物 ,在 496 nm波长处有最大吸收 ,其配合比为3∶ 1 .该方法的线性范围为 1 0 .0～ 2 0 0 μg/m L,相关系数为 0 .9995 ,摩尔吸光系数为 5 .11× 10 4L·mol-1·cm-1 ,平均回收率为 10 1 .2 %

A new method of spectrophotomertry of Nicotinamide by coordination reaction of Ferric chloride is studied. This basis is the acetamido group of Nicotinamide could react with hydroxylamine to form (N-hydroxyamino-amide) which give color reaction with Fe^(3+) and could be used to determinate Nicotinamide by spectrophotometry at the wavelength of 496 nm. The linear region was 10.0～200 μg/mL,R^2=0.999 5, ε=5.11×10~4 L·mol^(-1)·cm^(-1), RSD=1.2%,the average recovery was 101.2%.